![]() |
OpenMS
|
Identifies peptides in MS/MS spectra.
| pot. predecessor tools | → ProSE → | pot. successor tools |
|---|---|---|
| any signal-/preprocessing tool (in mzML or Bruker .d format) | IDFilter or any protein/peptide processing tool |
@em This search engine is mainly for educational/benchmarking/prototyping use cases. It lacks behind in speed and/or quality of results when compared to state-of-the-art search engines. @note Currently mzIdentML (mzid) is not directly supported as an input/output format of this tool. Convert mzid files to/from idXML using @ref TOPP_IDFileConverter if necessary. @note Open-search mode is automatically determined by the precursor mass tolerance: enabled when tolerance exceeds 1 Da or 1000 ppm. No explicit open-search parameter is needed. This is logged at runtime and recorded in the output search parameters as UserParam 'open_search'. @note Decoy handling: either enable '-Search:decoys' to generate decoys internally, or provide a FASTA database that already contains decoy proteins (e.g., from DecoyDatabase). In both cases, the decoy accession prefix must match '-Search:decoy_prefix' (default: "DECOY_"). <B>The command line parameters of this tool are:</B> @verbinclude TOPP_ProSE.cli <B>INI file documentation of this tool:</B> @htmlinclude TOPP_ProSE.html