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OpenMS
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Executes the top-hat filter to remove the baseline of an MS experiment.
| pot. predecessor tools | → BaselineFilter → | pot. successor tools |
|---|---|---|
| NoiseFilterSGolay, NoiseFilterGaussian | PeakPickerHiRes (or ID engines on MS/MS data) |
This nonlinear filter, known as the top-hat operator in morphological mathematics (see Soille, ''Morphological Image Analysis''), is independent of the underlying baseline shape. It is able to detect an over brightness even if the environment is not uniform. The principle is based on the subtraction of a signal from its opening (erosion followed by a dilation). The size the structuring element (here a flat line) being conditioned by the width of the lineament (in our case the maximum width of a mass spectrometric peak) to be detected.
The command line parameters of this tool are:
BaselineFilter -- Removes the baseline from profile spectra using a top-hat filter.
Full documentation: http://www.openms.de/doxygen/nightly/html/TOPP_BaselineFilter.html
Version: 3.6.0-pre-nightly-2026-03-06 Mar 7 2026, 01:46:19, Revision: c92c980
To cite OpenMS:
+ Pfeuffer, J., Bielow, C., Wein, S. et al.. OpenMS 3 enables reproducible analysis of large-scale mass spec
trometry data. Nat Methods (2024). doi:10.1038/s41592-024-02197-7.
Usage:
BaselineFilter <options>
Options (mandatory options marked with '*'):
-in <file>* Input raw data file (valid formats: 'mzML')
-out <file>* Output raw data file (valid formats: 'mzML')
-struc_elem_length <size> Length of the structuring element (should be wider than maximal peak width -
see documentation). (default: '3.0')
-struc_elem_unit <unit> Unit of 'struc_elem_length' parameter. (default: 'Thomson') (valid: 'Thomson',
'DataPoints')
-method <string> The name of the morphological filter to be applied. If you are unsure, use the
default. (default: 'tophat') (valid: 'identity', 'erosion', 'dilation', 'opening
', 'closing', 'gradient', 'tophat', 'bothat', 'erosion_simple', 'dilation_simple
')
Common TOPP options:
-ini <file> Use the given TOPP INI file
-threads <n> Sets the number of threads allowed to be used by the TOPP tool (default: '1')
-write_ini <file> Writes the default configuration file
--help Shows options
--helphelp Shows all options (including advanced)
INI file documentation of this tool:
This section lists all parameters supported by the tool. Parameters are organized into hierarchical subsections that group related settings together. Subsections may contain further subsections or individual parameters.
Each parameter entry contains the following information:
Parameter tags provide additional information about how a parameter is used. Some tags indicate whether a parameter is required or intended for advanced configuration, while others may be used internally by OpenMS or workflow tools.
Parameters highlighted as required must be specified for the tool to run successfully. Parameters marked as advanced allow fine-tuning of algorithm behavior and are typically not needed for standard workflows.