 |
OpenMS
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Resolves ambiguous annotations of features with peptide identifications.
| potential predecessor tools | → IDConflictResolver → | potential successor tools |
|---|---|---|
| IDMapper | TextExporter | |
| FeatureLinkerUnlabeled (or another feature grouping algorithm) | ProteinQuantifier |
The peptide identifications are filtered so that only one identification with a single hit (with the best score) is associated to each feature. (If two IDs have the same best score, either one of them may be selected.)
The the filtered identifications are added to the vector of unassigned peptides and also reduced to a single best hit.
This step may be useful before applying ProteinQuantifier, because features with ambiguous annotation are not considered for the quantification.
The command line parameters of this tool are:
IDConflictResolver -- Resolves ambiguous annotations of features with peptide identifications
Full documentation: http://www.openms.de/doxygen/nightly/html/TOPP_IDConflictResolver.html
Version: 3.3.0-pre-nightly-2024-11-20 Nov 21 2024, 02:34:56, Revision: decb5c8
To cite OpenMS:
+ Pfeuffer, J., Bielow, C., Wein, S. et al.. OpenMS 3 enables reproducible analysis of large-scale mass spec
trometry data. Nat Methods (2024). doi:10.1038/s41592-024-02197-7.
Usage:
IDConflictResolver <options>
Options (mandatory options marked with '*'):
-in <file>* Input file (data annotated with identifications) (val
id formats: 'featureXML', 'consensusXML')
-out <file>* Output file (data with one peptide identification
per feature) (valid formats: 'featureXML', 'consensus
XML')
-resolve_between_features <resolve_between_features> A map may contain multiple features with both identic
al (possibly modified i.e. not stripped) sequence
and charge state. The feature with the 'highest inten
sity' is very likely the most reliable one. When swit
ched on, the filter removes the sequence annotation
from the lower intensity features, thereby resolving
the multiplicity. Only the most reliable features
for each (possibly modified i.e. not stripped) sequen
ce maintain annotated with this peptide sequence.
(default: 'off') (valid: 'off', 'highest_intensity')
Common TOPP options:
-ini <file> Use the given TOPP INI file
-threads <n> Sets the number of threads allowed to be used by the
TOPP tool (default: '1')
-write_ini <file> Writes the default configuration file
--help Shows options
--helphelp Shows all options (including advanced)
INI file documentation of this tool:
1.9.1